spligak

8 ms·

1.
by spligak 3mo ago
Background on this question: CRISPR-Cas is a naturally occurring process in bacteria that is used to adapt to viral attacks. We've coopted the system for use in mammals. As far as I know a few labs in this space are operating under the
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by spligak 3mo ago
> I would deal with this by deciding how many cells I want to kill each day Yes, this is an approach. This starts to exceed my understanding of the problems the teams are facing - but there are concerns about the efficacy of Cas12a2-base
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by spligak 3mo ago
> How much overall has this costed you? For this project, seed capital low six figures. I am collaborating with family and friends, non-profits, and using doubling mechanisms available to me to at work to fund the very early speculative
4.
by spligak 3mo ago
> I don't think that more than sloppy accuracy is acceptable for any gene therapy Valid critiques of Cas12a2 must acknowledge the mechanistic differences between Cas9 and Cas12a2. There is no research to suggest Cas12a2 is "slo
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by spligak 3mo ago
> Are there any plans to move to in vivo? Yes! That's the next step. There wasn't a mouse model for my variant so they're building that, too. But in vivo testing should be underway this calendar year.
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by spligak 3mo ago
Done! Wrote a bit more in a comment above. Thank you for the interest.
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by spligak 3mo ago
I have now :) I replied to a sibling comment. Thank you for the nudge to share.
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by spligak 3mo ago
Thank you. I want to make sure you see the comment above - I think it was your comment that nudged me into writing it :) Happy to answer any other questions, here or over email (in my user profile). > other folks dealing with a tough dia
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by spligak 3mo ago
> Healthy cells will also get these nanoparticles, but without the triggering DNA sequence, the mRNA payload will remain inert and eventually will be degraded. This is my understanding as well.
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by spligak 3mo ago
> So how does Cas12a2 mitigate off-target effects? Others in this thread may be able to give a better analogy, but I'll try: Cas9 is like open heart surgery on millions of cells all at once. We know the specific outcome we want - a
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by spligak 3mo ago
I haven't written about it publicly, but I can elaborate here. I don't mind answering further questions about it even if you believe they'd make me uncomfortable - they won't. I've come to terms with what's hap
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by spligak 3mo ago
There are two major problems, delivery is one of them. Collateral damage of mass cell destruction leading to systemic inflammation is the other. The approach I'm reviewing now uses lipid nanoparticles (LNPs) for delivery. It isn't
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by spligak 3mo ago
You're correct about CRISPR Cas9. The off-target affects are difficult to manage. The paper describes Cas12a2. This is a different mechanism with discovery origins in - of all things - agriculture. It does not attempt in any way to rep
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by spligak 13y ago
The cost of cartridges are comparable to optical disks. However, LTO drives are non-trivial in terms of both mechanical complexity and cost. I can toss an optical tray into the recycle bin without batting an eye. Not true with LTO, and I su
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by spligak 13y ago
> I don't think the evidence we have is reliable enough to call someone "wildly misinformed" or "intentionally misleading", though. Perhaps not. However, my original challenge to rayiner's comment has produc
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by spligak 13y ago
You would think they'd have lifetime appointments as other federal courts do, but it's important to note FISC (and its review process) isn't the final stop on some Orwellian and diabolical train. It is very easy to get caught
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by spligak 13y ago
I question your assertion it is not an Article III [1] court. In fact, I think you are either wildly misinformed or being intentionally misleading. The FISA court of 11 judges is appointed by the Chief Justice of the United States. [2] It a
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by spligak 13y ago
The argument for a persons "right to die" may hold up to some degree. However, I believe the spirit of that sort of protection applies to those who are terminally ill. The idea being able to preserve a persons dignity and shorten the time s
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by spligak 14y ago
Slightly off-topic. Years ago when I was working at a small ISP in Columbia, SC I had lunch with Austin Meyer. He came in to upload the latest release of X-Plane to his FTP servers. We went across the street to get some shrimp and grits. Th
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by spligak 14y ago
It isn't a problem if you're speaking specifically about Cloudant. They co-locate clusters next to your app as best they can (as Mike has mentioned elsewhere). That makes the network you're running on critical, however. When we built out ou
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by spligak 14y ago
You cannot renounce your citizenship while in the country. The process requires you leave the US, enter an embassy or consulate and declare your intention to renounce. File some paperwork and you're no longer a citizen. The catch is you'll