koeng

8 ms·

1.
by koeng 3d ago
Recently got genomic DNA purification automated for yeast (plus have automated library prep for nanopore+ a promethion)! As part of the DNA purification task, I had to journey up to Healdsburg to buy some lysis enzyme (thank god for winemak
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by koeng 7d ago
I've worked in this field for many years. It's unfortunate that fears of AI have tarnished the field of synthetic cells so dramatically and yet so ignorantly. It's somewhat akin to believing we should ban charcoal because it
3.
Solod: Go can be a better C(solod.dev)
209 points by koeng 2mo ago|175 comments
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by koeng 4mo ago
Another thing I’m working on: homemade linen. Right now I just germinated a 4x8 bed with flax for fiber. The plan is to grow it for 100 days or so and then harvest, dry, ret, dry, and spin. I need a lot more to do anything serious, but I th
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by koeng 4mo ago
I’m smelting ore! I got into creating my own rings, and I’d really like to create one with ore I harvest myself. Gold is too hard and silver can be kinda dangerous, but malachite is pretty safe and I can just drive to Copperopolis to pick s
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by koeng 5mo ago
I met Craig about a year ago or so at a synthetic biology conference. Even though his institute was the one which created the first synthetic cell, he pretty much just talked about how disappointing it was that we couldn't engineer the
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by koeng 5mo ago
I do some synthetic biology as a hobby - genetically engineered a baker’s yeast to produce grape aroma and then baked bread with it, and gave it to like 100 people at an event I was at. Also do a few others - learned Esperanto (exclusively
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by koeng 7mo ago
if 10x the average amount of microplastics are showing changes that are approximately equivalent to hormonal or behavioral changes, it's not a significant factor to be worried about. There are many times where unblinded experiments are
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by koeng 7mo ago
eh, not that concerned
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by koeng 7mo ago
yes
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by koeng 7mo ago
Microplastics are bad. People are concerned that there are microplastics in your balls! And that this could epigenetically affect downstream generations. I want to test that theory with a real human, not an animal model. My plan: collect my
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by koeng 7mo ago
I work in DNA assembly and synthesis. Here is my take: They don't use oligo pools - "This capacity may be adapted to use large oligo pools to substantially reduce the cost per construct45 but requires further engineering to accoun
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by koeng 7mo ago
I am also a fan of SQLite. One of the best parts during development is how easy it is to spin up and spin down databases for full integration tests without containers or anything. It also simplifies backups, and is probably good enough.
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by koeng 8mo ago
I don’t think it’s legal for me to sell it (it is a genetically modified organism used in food), but I’d like to figure out if I can just give it away
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by koeng 8mo ago
keonigandall.com
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by koeng 8mo ago
Very neat! How do you standardize the gluten free mixes? I’ve been thinking about trying this since my mom is gluten free
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by koeng 8mo ago
Already answered there: I’m using bakers yeast, not lab yeast (store-bought S cerevisiae). It’s not haploid, often it’s tetraploid. HR doesn’t guarantee homozygous transformation. Same answer for electroporation vs spheroplasty. I’ve found
18.
by koeng 8mo ago
I’d recommend buying an Odin kit and just trying it. Doesn’t take THAT much to get into genetic engineering. The tough part is mostly the finesse in the simple things, like trying this in bakers yeast rather than lab yeast, or the genetic d
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by koeng 8mo ago
Eh, other than the electroporator I could probably do it for about $100-$200 bucks of equipment if I had a decent kitchen. Reagents probably about $300, but you can use em in a bunch of reactions, in aggregate down to like $50. The fundamen
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by koeng 8mo ago
I’ve only done grape so far. It’s on the verge of subtle vs unsubtle. If you’re real used to smelling yeast OR are a woman who has a strong sense of smell, you can smell it. Otherwise it’s just bread. It’s kinda unfair how much better women
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by koeng 8mo ago
I really need to do a write-up. I kinda just whip up the easiest path and do it. For example for the grape, I needed to knock out some tryptophan synthesis genes so I could redirect the bioflux. Problem is that in bakers yeast they have a w
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by koeng 8mo ago
I’m genetically engineering yeasts to make subtly flavored breads. I’ve already done grape aroma, now working on wintergreen. Also working on a red chamomile (using beat red biosynthesis). Just for fun. Red chamomile tea! The idea is to hav
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by koeng 9mo ago
Neat! Would this mainly be used for JavaScript servers running bun (ie, not end users)?
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by koeng 9mo ago
“The Panopticon is good”
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by koeng 10mo ago
I've been working on a sillier project lately. Green teeth! Lumina has made a probiotic strain that is able to, theoretically, prevent cavities. I don't care that much about, but I do think it is a neat strain that can likely colo
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by koeng 10mo ago
I don’t think Java and Rust were so ok with completely removing features. For example, in Zig 0.15 they completely overhauled the io, meaning all libraries now have to rewrite up usage. Just to make sure they did it right
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by koeng 11mo ago
95% for humans. Who failed to get the butter?
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by koeng 11mo ago
Very chill :) I do this at an industrial scale. It gets really annoying as you scale up to hundreds / thousands of different strains, all of which need pickable colonies. A serial dilution 3 or 4 times seems to always do the trick. Typ
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by koeng 11mo ago
We've known TALENs work for years. For example - https://pmc.ncbi.nlm.nih.gov/articles/PMC4817924/ - from 2015 I worked on a project many years ago to do RNA import into yeast mitochondria (and then hopefully
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by koeng 11mo ago
I've thought a lot about this! My main goal is to create a cloud lab that doesn't suck - ie, a remote lab that is actually useful for people, and a lot of these are relevant things. Let me run down the ideas I have for each 1. You

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