Add gene has a great guide as to what goes on at the molecular level: https://www.addgene.org/guides/crispr/ https://www.addgene.org/guides/crispr/ Essentially

Add gene has a great guide as to what goes on at the molecular level: https://www.addgene.org/guides/crispr/ https://www.addgene.org/guides/crispr/ Essentially you can design an rna molecular that contains a 20 nucleotide long sequence that can target your region of interest, with the caveat that there is a standard recognition sequence proximal to your sequence of interest (PAM sequence)