You're right that you don't need CRISPR for genome engineering of S. cerevisiae (baker's yeast) or E. coli. However, having worked with both prokaryotes and eu

You're right that you don't need CRISPR for genome engineering of S. cerevisiae (baker's yeast) or E. coli. However, having worked with both prokaryotes and eukaryotes it definitely makes life easier. During my PhD (about metabolic engineering of S. cerevisiae) CRISPR started to get traction. Before I could incorporate one change at a time. Using CRISPR/Cas9 I could do up to 6 (at different places). That's a big deal! I also PoC'ed CRISPR in a bacterium (C. glutamicum). Also there it opened up new avenues.